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KMID : 0545120040140010197
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 1 p.197 ~ p.201
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Cloning of pdh Genes Encoding Subunits of Pyruvate Dehydrogenase Complex from Lactobacillus reuteri ATCC 55739
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NAM, SU JIN
PARK, JAE-YONG/KIM, JUNG KON/HA, YEONG LAE/YUN, HAN DAE/KIM, JEONG HWAN
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Abstract
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A 2-D gel protein analysis of Lactobacillus reuteri ATCC 55739 produced spots corresponding to subunits of the pyruvate dehydrogenase complex, as identified by N-terminal protein sequencing. Oligonucleotide probes specific for the subunits of the pyruvate dehydrogenase complex were synthesized and used to screen a L. reuteri genomic library to clone the structural genes. Two positive clones were isolated and identified as having the same 2.2 kb insert. A pdhB encoding the β-subunit of El subunit (pyruvate dehydrogenase component) of the pyruvate dehydrogenase complex was located in the middle of the insert. Furthermore, a 5´ truncated pdhA encoding the a-subunit of the El subunit and a 3´ truncated pdhC encoding the E2 subunit (dihydrolipoamide acetyltransferase) were also located upstream and downstream of the pdhB, respectively.
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KEYWORD
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